Precautions for vitrification cryogenic liquid

vitrification cryogenic liquid is often used to solidify biological samples, but the following matters need to be paid attention to during use:

1. Safe operation: vitrification cryogenic liquid has a very low temperature. The commonly used vitrification cryogenic liquid is liquid nitrogen, and the temperature can reach minus 196°C. It is necessary to wear standard protective gloves, goggles and other protective equipment during operation to prevent liquid nitrogen from harming the skin and eyes.

2. Sealed container: vitrification cryogenic liquid should be stored in a sealed container to prevent liquid nitrogen evaporation and leakage. Sealed containers should have good sealing performance and be able to withstand the impact of low temperatures.

3. Prevent combustion: Liquid nitrogen can create a fire or explosion hazard when in contact with combustible materials. Therefore, when using vitrified refrigerant, keep away from fire sources and flammable materials to ensure the safety of the surrounding environment.

4. Avoid contact with skin: Liquid nitrogen has extremely low temperatures, and contact with skin can cause tissue frostbite. When using vitrification cryogenic liquid, direct contact with liquid nitrogen should be avoided and special tools or containers can be used for handling.

5. Ventilation environment: When operating vitrified refrigerant, ensure that the operating area has good ventilation facilities to prevent the gas generated by the evaporation of liquid nitrogen from causing harm to the human body.

In short, when using vitrification cryogenic liquid, users should follow relevant safety operating procedures to protect their own safety and ensure the safety of equipment and laboratories.