Nucleic acid molecular hybridization instrument uses and principles

Nucleic acid molecule hybridizer is used to detect the presence of complementary sequences in nucleic acid molecules, or to detect the presence of binding between two molecules, or to detect specific DNA sequences.

The principle of the nucleic acid molecular hybridizer is to use DNA molecular hybridization technology to position and detect the location and relative distance of a specific DNA sequence in chromosomes and genomes.

The nucleic acid molecule hybridization process mainly includes two steps: the first step is to denature two DNA single strands from different sources and melt them to form double strands; the second step is to combine one of the double strands with a known sequence The probe anneals to form a specific hybridized double strand. If the double-stranded sequence is completely consistent with the probe sequence, the molecular hybridization reaction is complete. During the reaction, biotin-labeled single-stranded DNA is added. If the probe sequence and biotin are complementary at the same site, a double-stranded DNA can be formed.

Nucleic acid molecular hybridization instrument can be used to detect antibodies in peripheral serum samples, which is helpful for clinical diagnosis of diseases. The principle is to use known fragments of genes (specific oligonucleotides) to hybridize with gene fragments in the sample to be tested (ie, pathogen-specific gene fragments), and then use methods such as autoradiography to display the presence or absence of radioactive isotopes. exist, thereby identifying specific gene fragments of the pathogen in the sample.