How to use nucleic acid molecular hybridizer

How to use the nucleic acid molecular hybridizer is as follows:

1. Pre-denaturation: Add 95°C pre-denaturation solution for 30 seconds .

2. Denaturation: Add 10×binding buffer for 30 seconds.

3. Annealing: Add specimen or probe, mix gently, and incubate at 55°C for 5 minutes.

4. Hybridization: Add hybridization buffer, mix gently, and incubate at 37°C for 2-4 hours.

5. Wash the plate: The process of washing the plate is: shake off the liquid on the plate → lightly print it with absorbent paper → soak in the washing liquid → gently shake the mixing solution to wash the plate → shake off the liquid on the plate → lightly print it with absorbent paper → Soak in the final washing solution → Gently shake the mixing solution to wash the plate → Shake off the liquid on the plate → Gently print dry with absorbent paper.

6. Color development: Add biotin-labeled secondary antibody, mix gently, and incubate at 37°C for 15 minutes.

7. Terminate the reaction: add stop solution, mix gently, and leave at room temperature for 10 minutes.

8. Washing: The plate washing process is: shake off the liquid on the plate → lightly print on absorbent paper → soak in the washing liquid → gently shake the mixing solution to wash the plate → shake off the liquid on the plate → lightly print on absorbent paper → Soak in the final washing solution → gently shake the mixing solution to wash the plate → shake off the liquid on the plate → lightly print on absorbent paper to dry.

9. Color development: Add substrate color development solution, mix gently, and leave at room temperature for 10 minutes.

10. Terminate the reaction: add stop solution, mix gently, and leave at room temperature for 10 minutes.

Note: Some of the above steps require high-temperature treatment, such as pre-denaturation, denaturation, annealing, hybridization, plate washing, color development, termination reaction, and termination reaction. When performing these high-temperature treatments, attention needs to be paid to controlling temperature and time to avoid DNA degradation and other side reactions. In addition, when washing the plate, you need to pay attention to cleaning it to avoid cross contamination.