Nucleic acid molecular hybridizer

Nucleic acid molecule hybridizer biomedical equipment is mainly used to detect nucleic acid sequences in samples. By hybridizing a nucleic acid probe with a known sequence to the nucleic acid molecules in the sample, it can detect the presence of a specific nucleic acid sequence in the sample. This technology is widely used in genetic diagnosis, genome analysis, pathogen detection and other fields.

In the process of nucleic acid molecule hybridization, the probe is an oligonucleotide or single-stranded DNA fragment with a known sequence. It is denatured and hybridized with the denatured nucleic acid molecule under high temperature conditions. At a certain temperature, the two interact through hydrogen bonds. Combined together to form a hybrid. This process requires high temperatures and is usually separated using denaturing polyacrylamide electrophoresis or agarose gel electrophoresis.

Next, the hybrid needs to be cooled so that it remains stable at low temperatures. At this time, if the sample contains the same or complementary nucleic acid sequence as the probe, they will continue to combine and form a hybrid. This process needs to be carried out under low temperature conditions and is usually performed using membrane hybridization or liquid phase hybridization.

Finally, hybrids can be specifically detected, often using radioisotope-labeled probes. This process can be detected by radioactivity counting or radioisotope scanners.

In short, the nucleic acid molecular hybridizer is a very useful device that can help researchers and doctors detect and analyze nucleic acid sequences more accurately to better understand genome structure and function, as well as diagnose and treat diseases.