Flow Cytometry Notes

1. Avoid too strong light: The flow cytometer is highly sensitive to the light of the sample, and too strong light may cause false alarms or data distortion. Therefore, it is necessary to avoid too strong light irradiating the sample during the experiment.

2. High requirements for sample preparation: Since the resolution of the flow cytometer is relatively high, impurities and noise in the sample may affect the experimental results, so special attention must be paid to sample preparation and quality requirements.

3. Data quality control: In order to ensure the accuracy of the experimental results, it is necessary to perform quality control on the data during the experiment. Common methods include filtering out abnormal data and conducting quality control experiments.

4. Instrument maintenance: Flow cytometer is a high-precision instrument that requires regular maintenance and calibration, which usually includes cleaning the instrument and replacing the reagent pressure tank.

5. Be familiar with instrument operation and data processing methods: Since the operation of the flow cytometer is relatively complicated, you need to be familiar with various parameters and experimental procedures. At the same time, you also need to have a certain understanding of the processing and analysis methods of the experimental results.