Function and usage of flow cytometer

Flow cytometer biochemical experiment instrument can analyze and measure single biological cells. Its functions and uses are as follows:

1. Quantitative analysis: It can be used to quantitatively analyze specific components in a sample, such as detecting the concentration of white blood cells or tumor cells in the blood.

2. Sorting and screening: Cell surface markers can be used to sort and screen out cell subpopulations with specific biological characteristics for subsequent research.

3. Single-cell analysis: Complex detection and analysis of single cells can be performed, such as measuring the gene expression profile, metabolite level, protein composition and function of single cells.

4. Study cell dynamics: Dynamic processes such as cell growth, division and death can be studied by continuously monitoring and tracking a large number of cells.

5. Control cell experimental conditions: By controlling parameters such as light source, temperature and flow rate in the flow cytometer, a stable environment and conditions can be provided for cell experiments.

Instructions:

1. Prepare flow cell suspension: collect cells by centrifugation, wash the cells with physiological saline or PBS to make the cell concentration appropriate (generally between 1×10^6 ~ 1×10^8/mL), add staining agent (such as PI, FITC, etc.) or antibody, and mix well.

2. Debug the instrument: turn on the power of the flow cytometer, start the software, and perform equipment calibration, light source calibration, etc.

3. Calibration: Place the standard particle suspension into the sample tube and calibrate the instrument to obtain the standard value.

4. Start the test: Take a small amount of cell suspension and add it to the sample tube to make the sample uniform. Turn on the laser light source, adjust the appropriate flow rate, and let the sample flow continuously into the detection chamber. The instrument analyzes the fluorescence intensity and scattered light of the cells through laser and photoelectric multi-channel detectors to obtain information such as cell number and fluorescence expression level.

5. Analyze data: Perform statistics, analysis, and drawing operations on the obtained data, such as cell classification, fluorescence expression level, cell proliferation rate, etc.