How to use disposable DNA immunoadsorption columns

1. Preparation: Prepare the required reagents, samples and instruments, make sure the experimental surface is clean and tidy, and wear gloves.

2. Sample processing: Add the sample to be processed into a centrifuge tube and centrifuge to remove impurities. If the sample is a cell extract or serum, conventional methods can be used for extraction and centrifugation.

3. Column preparation: Open the disposable DNA immunoadsorption column box and take out a column. Insert a collection tube or centrifuge tube into the small opening at the bottom of the column, making sure the bottom is sealed.

4. Column activation: Add 5-10 mL of column activation buffer to the column, and gently shake the column to completely activate the column. After the buffer has completely penetrated the column, remove the bottom collection tube or centrifuge tube and discard it.

5. Sample loading: Add the centrifuged sample buffer to the activated column to ensure that the sample completely covers the column medium. Samples can be slowly added to the column using a pipette or dropper.

6. Column elution: After loading the sample, add the elution buffer to the column immediately and allow it to flow through the column. The volume of the elution buffer is usually 1-5 times the volume of the sample buffer, which is determined based on the experimental requirements.

7. Column storage: Remove the collection tube or centrifuge tube containing the elution product and conduct further analysis or storage. Discard used columns.

8. Cleaning step: Reinsert the small opening at the bottom of the column into a collection tube or centrifuge tube, then add a small amount of wash buffer and shake the column to clean the column media. Remove the collection tube or centrifuge tube and discard.

The above is the method of using the disposable DNA immunoadsorption column. Minor adjustments can be made as needed according to the actual situation. Before proceeding, it is recommended to refer to the detailed instructions for the purchased column to understand its specific usage and operating procedures.