Real-time quantitative PCR instrument features and uses

Features of real-time quantitative PCR machines include:
1. High detection sensitivity: a very small amount of template DNA can be detected, and the minimum detection amount can reach 10 to 10.
2. Strong specificity: non-specific product peaks are eliminated, so there will be no false positives.
3. Good repeatability: small inter-batch and intra-batch variation, small coefficient of variation, and good repeatability.
4. Convenient and fast analysis: multiple samples can be tested at the same time.
5. Easy to operate: mix the template after adding it, keep it at 37°C for 1 minute, and then start real-time monitoring.
6. Real-time quantitative analysis: Through PCR amplification of standards and samples, quantification of standards and measurement of Ct values, the transcription level of the target gene can be determined.

The real-time quantitative PCR instrument is mainly used to detect the number of times a specific gene appears in the sample to be tested, and uses high-resolution denaturing gradient gel electrophoresis dendrogram to sequence the PCR product using a polymerase chain reaction product analysis method.