Real-time quantitative PCR machine

The real-time quantitative PCR instrument can monitor the reaction in real time and obtain a series of corresponding data through a series of photoelectric reactions when the copy number of each template changes. And during the DNA replication process, template DNA can be quantitatively detected.

There are many classification methods for real-time quantitative PCR instruments. The following are two common classification methods:
1. According to technical principles:
(1) TaqMan monitoring method: The principle of this method is to add a fluorescent probe to the PCR reaction system. The labeled fluorescent group of the probe will be continuously released during the PCR amplification process and be detected by the real-time PCR instrument. detection. When the PCR product accumulates to a certain amount, it hybridizes with the probe and generates a fluorescent signal. The instrument automatically records the amplification curve and CT value, and finally calculates the copy number of the target gene.

(2) Others include SYBR Green method, Rox dye method, two-photon excitation system, and molecular beacons.

2. According to application form:

(1) PCR instrument for cDNA synthesis: used for cDNA first strand synthesis, sample addition after cDNA first strand synthesis, PCR amplification after cDNA first strand synthesis, etc.

(2) PCR instrument for PCR amplification: including 96 or 384-well plate sample chamber, PCR amplification instrument, PCR amplification mixing chamber, circulation module, temperature control system and fluorescence detection system.

(3) Real-time fluorescence quantitative PCR instrument: This new type of PCR instrument can add fluorescent groups to the PCR reaction system and reflect the accumulation of PCR products through the detection of fluorescent signals.