petri dish

Petri dish definition

designed in 1887 by Julius Richard Petri (1852-1921), a bacteriologist who worked under   the German biologist Robert Koch , so it is also called "Petri". Inside the dish". Petri dishes are fragile and breakable, so be careful and handle them with care when cleaning and handling them. It is best to clean the Petri dishes after use in time and store them in a safe and fixed location to prevent damage or falling.

Petri dish composition

A bottom and a lid. The bottom is small and the lid is big . The bottom of the dish is used for culture.

Petri dish cleaning steps

Generally, it goes through four steps: soaking, brushing, pickling , and cleaning.

1. Soaking: New or used glassware should be soaked in clean water first to soften and dissolve attachments . Before use, new glassware must be briefly washed with tap water, and then soaked in 5% hydrochloric acid overnight; used glassware often has a large amount of protein and grease attached, which is difficult to wash off after drying, so it should be immersed in clean water immediately after use and prepared for brushing. .

2. Scrubbing: Put the soaked glassware into detergent water and scrub repeatedly with a soft brush. Do not leave any dead ends and prevent damage to the surface finish of the vessel . Wash the clean glassware, dry it, and prepare it for pickling.

3. Pickling: Pickling is to soak the above-mentioned utensils in a cleaning solution , also known as acid solution, to remove possible residual substances on the surface of the utensils through the strong oxidation of the acid solution. Pickling should not be less than six hours, usually overnight or longer. Be careful with the utensils.

       4. Rinse: The utensils after scrubbing and pickling must be fully rinsed with water. Whether the utensils are rinsed clean after pickling directly affects the success or failure of cell culture . When washing pickled utensils by hand, each utensil must be "watered and emptied" repeatedly at least 15 times, and finally soaked in double-distilled water 2-3 times, dried or dried, and then packaged for later use.