Creatinine Analyzer Principle

The principle of creatinine analysis instrument is mainly based on fluorescence method and electrode method.

1. Fluorescence method: After installing a laser excitation source, a light detector, a computer processing system and software on a fluorescence photometer, a laser fluorescence immunoassay analyzer can be established. Its working principle is based on the combination of fluorescence immunoassay technology and fluorescence photometer. It uses specific antibodies to react with corresponding antigens to form a complex. After adding fluorescently labeled specific antibodies, the complex reacts with fluorescent antibodies to form a new fluorescent complex. In the newly formed fluorescent complex, both fluorescent antibodies and antigens are directionally aggregated to the newly formed fluorescent complex, which enhances the fluorescence intensity. Finally, the fluorescence intensity is measured by a fluorescence photometer and converted into creatinine concentration by a computer system.

2. Electrode method: The instrument uses the principle of electrode method to measure blood creatinine. Before measurement, the sample and standard solution pass through the electrodes in the electrode chamber, and the ions in the solution will stimulate a potential diagram. Different ions have different reference electrodes and ion pairs, and each ion has a specific reference electrode and ion pair. When the potentials in these electrode chambers are measured, the computer calibrates and calibrates by comparing these potentials and obtains the measurement results.