Precautions for fully automatic dual-label fluorescence reader

When using a fully automated dual-label fluorescence reader, the following are some considerations:

1. Keep the instrument clean: Clean the instrument surface and optical components regularly to ensure accurate and clear fluorescence signals.

2. Use the correct labeling reagent: Make sure you use the correct fluorescent labeling reagent that is compatible with your instrument to get the best reading results.

3. Sample processing: During sample processing, try to avoid contamination or damage to reagents and specimens. Make sure the specimen is stable to avoid data distortion.

4. Set the threshold correctly: According to the characteristics of the sample and reading requirements, set the threshold correctly to distinguish positive and negative signals. Thresholds that are too high or too low can lead to misinterpretation of results.

5. Regular calibration: According to the instrument's user manual, perform regular calibration to ensure the accuracy and consistency of the instrument.

6. Pay attention to safety: When using fluorescent labeling reagents, follow relevant safety operating procedures, including wearing appropriate personal protective equipment and avoiding contact or inhalation of the reagents.

7. Adjust the light source and detector: Make sure the light source and detector are adjusted correctly to obtain optimal differentiation of fluorescence signal from background noise.

8. Read the results carefully: When reading the results, carefully observe and understand the positive and negative signals of the markers to avoid misinterpretation of the results.

9. Sample preservation: As needed, samples are properly preserved and labeled for subsequent analysis and verification.

10. Standardized operations: To ensure that reading results performed by different operators and at different time points are comparable and consistent, this can be achieved by using standardized operating procedures and quality control samples.