How to use the quantitative detection kit

The method of using the quantitative detection kit varies depending on the type of kit. The general steps are as follows:

1. Read the instructions carefully: It is recommended to read the user manual of the kit to understand the detection principle, operation process, judgment criteria, precautions and other information.
2. Prepare the required reagents and samples: According to the operation manual, prepare the required reagents, such as buffer, cleaning solution, etc. At the same time, ensure that the samples (such as blood, serum, cell culture medium, etc.) are properly prepared.
3. Confirm the testing environment: According to the requirements of the test kit, ensure that the experimental operation is carried out in a suitable environment, such as constant temperature, sterility, etc.
4. Add sample: Add the sample to the designated location in the kit according to the operating manual.
5. Add reagents: Add buffer, wash solution, and enzyme conjugate according to the steps. Make sure to add the required reagents correctly.
6. Incubation: Incubate the entire kit to ensure that all reagents and samples can react fully. Perform appropriate incubation and heat preservation according to the steps in the operation manual.
7. Washing: Before the color development reaction, the kit needs to be washed several times to remove irrelevant reaction substances. Follow the steps in the manual for washing.
8. Color development and termination: According to the operating manual, add color developer and stop solution to complete quantitative detection.
9. Observe the results: After an appropriate period of time (usually several minutes to half an hour), observe the reaction results of the kit. Make judgments based on the user manual of the kit and record them.

Please note that the above steps are for reference only. The specific methods should be followed in accordance with the user manual of the kit. In addition, when using the quantitative detection kit, be sure to pay attention to safety precautions and quality control requirements to ensure the accuracy and reliability of the results.