Microinjection microscope functions and how to use it

Microinjection microscopes are mainly used for various physiological, biochemical and molecular biology studies on living cells, such as: infusion research on biological macromolecules such as $DNA$, $RNA$, and proteins; research on early embryonic cells and differentiation and development. Research; dynamic research on calcium ion plasma in living cells; research on the signal transduction pathways of second messengers in living cells; research on the fertilization process of mammalian sperm, etc.

Microinjection technology is an operating technology that injects trace amounts of substances such as amino acids, nucleic acids, drugs, etc. into living bodies and living cells. It is performed under a microscope, therefore, it is also called microinjection.

Microinjection technology is an operating technology that injects trace amounts of substances such as amino acids, nucleic acids, drugs, etc. into living bodies and living cells. It is performed under a microscope, therefore, it is also called microinjection.

Steps:

1. Adjust the appropriate distance (100-150mm) under the microscope so that the distance between the injection head and the cells is 10-15mm.

2. Turn on the injection needle switch to create negative pressure in the needle and suck the required solution into the needle.

3. Pull out the needle, move the needle tip above the cells (about 1mm away from the cells), and insert the needle tip between the cells to make contact with the cell membrane.

4. Slowly inject all the liquid in the injection needle into the cells.

5. After the injection is completed, immediately insert the injection head into the cell and turn off the injection needle switch.