How to use micromanipulation fluid

The specific steps for sperm micromanipulation using micromanipulation fluid are as follows:

1. Wash the sperm: Under a microscope, use a small dropper or pipette to collect a drop of sperm, add it to a centrifuge tube containing 9 ml of washing solution, and gently blow and pipette several times to suspend the sperm in the washing solution.

2. Wash sperm: inject the washed sperm suspension into another centrifuge tube and centrifuge for 10 minutes (2000r/min).

3. Add micromanipulation fluid: Add 400ul of micromanipulation fluid to the centrifuge tube and gently pipette to mix evenly.

4. Micromanipulation: Under a microscope, use a micropipette to take a drop of sperm suspension, move it to the stage, use the micromanipulation system to adjust the magnification, and use a rubber-tipped dropper in the micromanipulation fluid to mix the sperm with a trace amount of culture medium.

5. Collect sperm after operation: use a micropipette to aspirate the trace culture medium containing sperm, inject it into a centrifuge tube containing 9ml washing solution, and gently blow it evenly.

Please note that sperm micromanipulation is a technically demanding procedure that requires professional training and practice to master. If you require sperm micromanipulation services, it is recommended that you consult a professional laboratory or medical institution.